Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 456731
Title Reduced seed germination in Arabidopsis over-expressing SWI/SNF2 ATPase genes
Author(s) Leeggangers, H.A.C.F.; Folta, A.; Muras, A.; Nap, J.P.H.; Mlynarova, L.
Source Physiologia Plantarum 153 (2015)2. - ISSN 0031-9317 - p. 318 - 326.
DOI https://doi.org/10.1111/ppl.12231
Department(s) Laboratory of Plant Physiology
Laboratory of Molecular Biology
BIOS Applied Bioinformatics
EPS-4
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) thaliana - dormancy - transcription - maturation - stress - arrest - growth - rna
Abstract In the life of flowering plants, seed germination is a critical step to ensure survival into the next generation. Generally the seed prior to germination has been in a dormant state with a low rate of metabolism. In the transition from a dormant seed to a germinating seed, various epigenetic mechanisms play a regulatory role. Here, we demonstrate that the over-expression of chromatin remodeling ATPase genes (AtCHR12 or AtCHR23) reduced the frequency of seed germination in Arabidopsis thaliana up to 30% relative to the wild-type seeds. On the other hand, single loss-of-function mutations of the two genes did not affect seed germination. The reduction of germination in over-expressing mutants was more pronounced in stress conditions (salt or high temperature), showing the impact of the environment. Reduced germinations upon over-expression coincided with increased transcript levels of seed maturation genes and with reduced degradation of their mRNAs stored in dry seeds. Our results indicate that repression of AtCHR12/23 gene expression in germinating wild-type Arabidopsis seeds is required for full germination. This establishes a functional link between chromatin modifiers and regulatory networks towards seed maturation and germination.
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