Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 477110
Title A strategy for developing representative germplasm sets for systematic QTL validation, demonstrated for apple, peach, and sweet cherry
Author(s) Peace, C.P.; Luby, J.; Weg, W.E. van de; Bink, M.C.A.M.; Iezzoni, A.F.
Source Tree Genetics and Genomes 10 (2014)6. - ISSN 1614-2942 - p. 1679 - 1694.
DOI https://doi.org/10.1007/s11295-014-0788-z
Department(s) Plant Breeding
Biometris
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) x domestica borkh. - marker-assisted selection - fire blight resistance - breeding program - population-structure - pyrus-communis - fruit firmness - map position - md-acs1 - gene
Abstract Horticultural crop improvement would benefit from a standardized, systematic, and statistically robust procedure for validating quantitative trait loci (QTLs) in germplasm relevant to breeding programs. Here, we describe and demonstrate a strategy for developing reference germplasm sets of perennial, clonally propagated crops, especially those with long juvenile periods. Germplasm is chosen to efficiently represent important members of larger pedigree-connected genepools. To facilitate validation of multiple QTLs, genome-wide representation of alleles is optimized for designated important breeding parents (IBPs) by estimating average allelic representation in relatives. The strategy and arising principles were demonstrated in a simulated germplasm set. Strong statistical power can be achieved with a carefully chosen germplasm set composed of IBPs, their numerous unselected progenies and close relatives, and all available founders and intermediate ancestors. Crop Reference Sets were developed in the marker-assisted breeding (MAB)-enabling “RosBREED” project as a base resource for QTL validation in US breeding germplasm of apple (Malus × domestica), peach (Prunus persica), and sweet cherry (Prunus avium) consisting of 467, 452, and 268 individuals, respectively. These sets adequately represent the most designated IBPs, have distinct advantages for QTL validation over other germplasm arrangements of equal size, and are recommended as a base resource for QTL validation by breeders of these US crops. The strategy described here can be used to develop efficient reference germplasm sets suiting other breeding genepools or to calculate the statistical power for QTL validation of germplasm sets already established.
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