Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 477856
Title The actin cytoskeleton in Phytophthora infestans in free living mycelium and infection
Author(s) Meijer, H.J.G.; Kots, K.; Hua, C.; Govers, F.
Source In: Book of Abstracts Oomycete Molecular Genetics Network Meeting. - - p. 14 - 14.
Event 2014 OMGN Meeting, Norwich, UK, 2014-07-02/2014-07-04
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2014
Abstract The actin cytoskeleton is a dynamic but well organized intracellular framework that is indispensable for proper functioning of eukaryotic cells. The plethora of functions ranges from facilitating transport of vesicles, muscle contraction, formation of contractile rings, nuclear segregation, endocytosis and facilitating apical cell expansions. To visualize the dynamics of the actin cytoskeleton in the oomycete Phytophthora infestans we generated transformants expressing the actin binding peptide Lifeact-eGFP as an in vivo marker. Fluorescence microscopy revealed that all actin structures in P. infestans hyphae, including actin filament cables and actin filament plaques, are cortically localized. The actin filament plaques are distributed over the hyphae but their presence in the hyphal tip is related to hyphal tip growth. They are nearly immobile with an extremely long lifespan when compared to that of actin patches in other eukaryotes and their disassembly is not accompanied with internalization and the formation of endocytic vesicles as is the case with actin patches in yeast. We also investigated the in vivo actin cytoskeleton dynamics during growth in a low nutrient environment and during early stages of pathogenesis such as appressorium formation and infection. Our observations suggest that the actin cytoskeleton performs a variety of regulatory roles during distinct life stages of P. infestans. Future efforts will focus at identifying interactors and key regulators of the actin cytoskeleton and pinpoint features in the actin network that are unique for oomycetes.
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