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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 477869
Title Genomic rearrangements drive effector evolution in Verticillium dahliae
Author(s) Faino, L.; Seidl, M.F.; Berg, G.C.M. van den; Datema, E.; Janssen, A.; Wittenberg, A.; Thomma, B.P.H.J.
Source In: Book of Abstracts XVI International Congress on Molecular Plant-Microbe Interactions. - - p. 57 - 57.
Event XVI International Congress on Molecular Plant-Microbe Interactions, Rhodes, Greece, 2014-07-06/2014-07-10
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2014
Abstract P114 - Verticillium dahliae is the causal agent of tomato vascular wilt disease. While race 1 isolates express the Ave1 effector that is recognized by the tomato Ve1 immune receptor, race 2 isolates lacks this effector that was identified based on comparative genomics. Comparative genomics further revealed extensive genomic rearrangements between V. dahliae strains leading to lineage-specific regions involved in virulence and niche adaptation. To understand the role of rearrangements in effector evolution between V. dahliae strains, we re- sequenced the ~37 Mb genome of V. dahliae strain JR2 using PacBio and assembled a gapless genome which was aligned to the assembly of strain Ls17 and synteny breakpoints were identified. Subsequently, we assessed the genomic localizations of in planta-induced genes and we prove that virulence genes localize in lineage- specific regions and these regions are in close proximity to genomic rearrangements. Furthermore, we focused on the well-characterized effector Ave1 which localizes in a region were rearrangements occur. Aligning of Illumina reads of 10 V. dahliae strains to the gapless JR2 genome revealed that the Ave1 locus was lost multiple times during evolution.
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