Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 477889
Title A novel in vitro infection system to study Phytophthora-host interactions
Author(s) Schoina, C.; Govers, F.; Bouwmeester, K.
Source In: Book of Abstracts COST 2014 - 2nd Annual Conference of the SUSTAIN Action. - - p. 17 - 17.
Event 2nd Annual Conference of the SUSTAIN Action, Zakopane, Poland, 2014-10-15/2014-10-17
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2014
Abstract One of the most devastating plant diseases world wide is late blight on potato and tomato caused by the oomycete pathogen Phytophthora infestans. During the early biotrophic phase of infection, Phytophthora penetrates host tissue and thereafter forms specialized feeding structures called haustoria. Here, effectors produced by the pathogen, are transferred into the host cells to manipulate the host cell machinery thereby suppressing plant defense. Therefore, studying the interface between the host and the pathogen at the early stages of infection is of great interest. An important drawback when studying the Phytophthora-host interaction in leaves is the lack of synchronization of the infection process. For this purpose, a new in vitro infection system was established, in which MsK8 tomato cell suspensions were challenged with zoospores of different Phytophthora species. Here we show that P. infestans infects MsK8 cells in a similar fashion as tomato leaf tissue. In contrast, other Phytophthora species that are not pathogenic on tomato could not penetrate the MsK8 cells. Expression analyses of Phytophthora effector and tomato defense genes and various histological assays were performed to monitor Phytophthora-MsK8 interactions in more detail. In addition, multi-omic datasets were genereated (i.e., transcriptome, metabolome, proteome), and are currently integretated and analyzed in a systems biology approach to elucidate the essential processes during Phytophthora-MsK8 interaction. The use of this novel infection system allows simplification and synchronization of the infection process, and is expected to provide a more detailed insight into Phytophthora-host interactions.
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