Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 478651
Title Chromosomal organizations of major repeat families on potato (Solanum tuberosum) and further exploring in its sequenced genome
Author(s) Tang, X.; Datema, E.; Olortegui Guzman, M.C.; Boer, J.M. de; Eck, H.J. van; Bachem, C.W.B.; Visser, R.G.F.; Jong, H. de
Source Molecular Genetics and Genomics 289 (2014)6. - ISSN 1617-4615 - p. 1307 - 1319.
DOI https://doi.org/10.1007/s00438-014-0891-8
Department(s) Plant Breeding
Laboratory of Plant Physiology
Plant Breeding
Laboratory of Genetics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) ty1-copia group retrotransposons - species-specific sequences - repeated dna-sequences - in-situ hybridization - somatic hybrids - lycopersicon-esculentum - satellite repeat - repetitive dna - transposable elements - metaphase chromosomes
Abstract One of the most powerful technologies in unraveling the organization of a eukaryotic plant genome is high-resolution Fluorescent in situ hybridization of repeats and single copy DNA sequences on pachytene chromosomes. This technology allows the integration of physical mapping information with chromosomal positions, including centromeres, telomeres, nucleolar-organizing region, and euchromatin and heterochromatin. In this report, we established chromosomal positions of different repeat fractions of the potato genomic DNA (Cot100, Cot500 and Cot1000) on the chromosomes. We also analysed various repeat elements that are unique to potato including the moderately repetitive P5 and REP2 elements, where the REP2 is part of a larger Gypsy-type LTR retrotransposon and cover most chromosome regions, with some brighter fluorescing spots in the heterochromatin. The most abundant tandem repeat is the potato genomic repeat 1 that covers subtelomeric regions of most chromosome arms. Extensive multiple alignments of these repetitive sequences in the assembled RH89-039-16 potato BACs and the draft assembly of the DM1-3 516 R44 genome shed light on the conservation of these repeats within the potato genome. The consensus sequences thus obtained revealed the native complete transposable elements from which they were derived
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