Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 479391
Title Crystal structure of the CRISPR RNA–guided surveillance complex from Escherichia coli
Author(s) Jackson, R.N.; Golden, S.M.; Erp, P.B.; Carter, J.; Westra, E.R.; Brouns, S.J.J.; Oost, J. van der; Terwilliger, T.C.; Read, R.J.; Wiedenheft, B.
Source Science 345 (2014)6203. - ISSN 0036-8075 - p. 1473 - 1479.
DOI https://doi.org/10.1126/science.1256328
Department(s) Microbiology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) bacterial immune-system - processes pre-crrna - thermus-thermophilus - cas systems - interference complex - target recognition - antiviral defense - seed sequence - dna - cascade
Abstract Clustered regularly interspaced short palindromic repeats (CRISPRs) are essential components of RNA-guided adaptive immune systems that protect bacteria and archaea from viruses and plasmids. In Escherichia coli, short CRISPR-derived RNAs (crRNAs) assemble into a 405-kilodalton multisubunit surveillance complex called Cascade (CRISPR-associated complex for antiviral defense). Here we present the 3.24 angstrom resolution x-ray crystal structure of Cascade. Eleven proteins and a 61-nucleotide crRNA assemble into a seahorse-shaped architecture that binds double-stranded DNA targets complementary to the crRNA-guide sequence. Conserved sequences on the 3' and 5' ends of the crRNA are anchored by proteins at opposite ends of the complex, whereas the guide sequence is displayed along a helical assembly of six interwoven subunits that present five-nucleotide segments of the crRNA in pseudo–A-form configuration. The structure of Cascade suggests a mechanism for assembly and provides insights into the mechanisms of target recognition.
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