Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 480017
Title Live Faecalibacterium prausnitzii in an apical anaerobic model of the intestinal epithelial barrier
Author(s) Ulluwishewa, D.; Anderson, R.C.; Young, W.; McNabb, W.C.; Baarlen, P. van; Moughan, P.J.; Wells, J.M.; Roy, N.C.
Source Cellular Microbiology 17 (2015)2. - ISSN 1462-5814 - p. 226 - 240.
DOI https://doi.org/10.1111/cmi.12360
Department(s) Host-Microbe Interactomics
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) necrosis-factor-alpha - crohns-disease - fusobacterium-prausnitzii - celiac-disease - hypoxia - permeability - expression - microbiota - diversity - inhibition
Abstract Faecalibacterium prausnitzii, an abundant member of the human commensal microbiota, has been proposed to have a protective role in the intestine. However, it is an obligate anaerobe, difficult to co-culture in viable form with oxygen-requiring intestinal cells. To overcome this limitation, a unique apical anaerobic model of the intestinal barrier, which enabled co-culture of live obligate anaerobes with the human intestinal cell line Caco-2, was developed. Caco-2 cells remained viable and maintained an intact barrier for at least 12¿h, consistent with gene expression data, which suggested Caco-2 cells had adapted to survive in an oxygen-reduced atmosphere. Live F.¿prausnitzii cells, but not ultraviolet (UV)-killed F.¿prausnitzii, increased the permeability of mannitol across the epithelial barrier. Gene expression analysis showed inflammatory mediators to be expressed at lower amounts in Caco-2 cells exposed to live F.¿prausnitzii than UV-killed F.¿prausnitzii, This, consistent with previous reports, implies that live F.¿prausnitzii produces an anti-inflammatory compound in the culture supernatant, demonstrating the value of a physiologically relevant co-culture system that allows obligate anaerobic bacteria to remain viable.
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