Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 481038
Title Toolkit for Visualization of the Cellular Structure and Organelles in Aspergillus niger
Author(s) Buren, E.B.J. ten; Karrenbelt, M.A.P.; Lingemann, M.; Chordia, S.; Deng, Y.; Hu, J.J.; Verest, J.M.; Wu, V.; Bello Gonzalez, T.D.G.; Heck, R.G.A. van; Odoni, D.I.; Schonewille, T.; Straat, L. van der; Graaff, L.H. de; Passel, M.W.J. van
Source ACS synthetic biology 3 (2014)12. - ISSN 2161-5063 - p. 995 - 998.
DOI https://doi.org/10.1021/sb500304m
Department(s) Microbiology
Systems and Synthetic Biology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) gene - hyphae
Abstract Aspergillus niger is a filamentous fungus that is extensively used in industrial fermentations for protein expression and the production of organic acids. Inherent biosynthetic capabilities, such as the capacity to secrete these biomolecules in high amounts, make A. niger an attractive production host. Although A. niger is renowned for this ability, the knowledge of the molecular components that underlie its production capacity, intercellular trafficking processes and secretion mechanisms is far from complete. Here, we introduce a standardized set of tools, consisting of an N-terminal GFP-actin fusion and codon optimized eforRed chromoprotein. Expression of the GFP-actin construct facilitates visualization of the actin filaments of the cytoskeleton, whereas expression of the chromoprotein construct results in a clearly distinguishable red phenotype. These experimentally validated constructs constitute the first set of standardized A. niger biomarkers, which can be used to study morphology, intercellular trafficking, and secretion phenomena.
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