Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 482960
Title Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate.
Author(s) Mujawar, L.H.; Kuerten, J.G.M.; Siregar, D.P.; Amerongen, A. van; Norde, W.
Source RSC Advances : An international journal to further the chemical sciences 4 (2014)37. - ISSN 2046-2069 - p. 19380 - 19388.
DOI https://doi.org/10.1039/C4RA01327A
Department(s) Physical Chemistry and Colloid Science
BBP Bioconversion
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) experimental-verification - protein microarrays - dna microarrays - contact-angle - evaporation - surfaces - drops - performance - fabrication - adsorption
Abstract During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain” spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e.,
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