Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 483042
Title A SpoT polymorphism correlates with chill stress survival and is prevalent in clinical isolates of Campylobacter jejuni
Author(s) Nierop Groot, M.N.; Boer, A.G. de; Pelt, W. van; Hulst-van Arkel, M.C. van der; Leeuw, P.; Widjaja, H.C.A.; Smits, M.A.; Wal, F.J. van der
Source Poultry Science 93 (2014)11. - ISSN 0032-5791 - p. 2900 - 2909.
DOI https://doi.org/10.3382/ps.2014-04055
Department(s) Food Technology
Infection Biology
CVI - Divisie Bacteriologie en TSE's
Animal Nutrition
Wageningen Livestock Research
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) virulence-associated phenotypes - htra degp protein - chicken carcasses - escherichia-coli - broiler meat - poultry - colonization - strains - netherlands - expression
Abstract Resistance of Campylobacter jejuni to environmental stress is regarded as a risk factor for the transmission of C. jejuni from poultry or poultry products to humans. So far, the mechanisms underlying the capacity of C. jejuni to survive environmental stress conditions are not fully understood. In this study, we searched for polymorphisms in C. jejuni genes, potentially involved in resistance to chill stress. To this end, we assessed 3 groups of C. jejuni isolates (clinical, retail chicken meat, and feces) for survival of experimentally induced chill stress. For each isolate we sequenced 3 genes encoding the C. jejuni sigma factors FliA, RpoD, and RpoN as well as the genes for the transcriptional regulator SpoT and the periplasmic protein HtrA. Data suggest a higher prevalence of a specific polymorphism in spoT in clinical isolates compared with poultry meat or farm isolates. Moreover, this genotype correlated with enhanced survival of chill stress. The observation that the prevalence of this SNP is relatively high in clinical isolates, which most likely have been exposed to multiple forms of stress, suggest that this SNP may be a biomarker for enhanced survival of stress.
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