Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 483766
Title Structural bisphenol analoques differentially target steroidogenesis in murine MA-10 Leydig cells as well as the glucocorticoid receptor
Author(s) Roelofs, M.J.E.; Berg, M. van den; Bovee, T.F.H.; Piersma, A.H.; Duursen, M.B.M. van
Source Toxicology 329 (2015). - ISSN 0300-483X - p. 10 - 20.
DOI https://doi.org/10.1016/j.tox.2015.01.003
Department(s) AFSG Quality in Chains
BU Toxicology, Novel Foods & Agrochains
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) endocrine-disrupting chemicals - tetrabromobisphenol-a tbbpa - brominated flame retardants - one-generation reproduction - in-vitro - fetal testis - exogenous progesterone - gene-expression - risk-assessment - united-states
Abstract Although much information on the endocrine activity of bisphenol A (BPA) is available, a proper human hazard assessment of analogues that are believed to have a less harmful toxicity profile is lacking. Here the possible effects of BPA, bisphenol F (BPF), bisphenol S (BPS), as well as the brominated structural analogue and widely used flame retardant tetrabromobisphenol A (TBBPA) on human glucocorticoid and androgen receptor (GR and AR) activation were assessed. BPA, BPF, and TBBPA showed clear GR and AR antagonism with IC50 values of 67 µM, 60 µM, and 22 nM for GR, and 39 µM, 20 µM, and 982 nM for AR, respectively, whereas BPS did not affect receptor activity. In addition, murine MA-10 Leydig cells exposed to the bisphenol analogues were assessed for changes in secreted steroid hormone levels. Testicular steroidogenesis was altered by all bisphenol analogues tested. TBBPA effects were more directed towards the male end products and induced testosterone synthesis, while BPF and BPS predominantly increased the levels of progestagens that are formed in the beginning of the steroidogenic pathway. The MA-10 Leydig cell assay shows added value over the widely used H295R steroidogenesis assay because of its fetal-like characteristics and specificity for the physiologically more relevant testicular ¿4 steroidogenic pathway. Therefore, adding an in vitro assay covering fetal testicular steroidogenesis, such as the MA-10 cell line, to the panel of tests used to screen potential endocrine disruptors, is highly recommendable.
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