Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 485806
Title An updated conventional- and a novel GM potato late blight R gene differential set for virulence monitoring of Phytophthora infestans
Author(s) Suxian Zhu, Suxian; Vossen, J.H.; Bergervoet-van Deelen, J.E.M.; Nijenhuis, M.A.; Kodde, L.P.; Kessel, G.J.T.; Vleeshouwers, V.G.A.A.; Visser, R.G.F.; Jacobsen, E.
Source Euphytica 202 (2015)2. - ISSN 0014-2336 - p. 219 - 234.
DOI https://doi.org/10.1007/s10681-014-1276-0
Department(s) Plant Breeding
PRI Biodiversity and Breeding
PBR Ornamentals, tissue culture and gene transfer
Food, Health & Consumer Research
Centre for Ecosystem Studies
Entomology & Disease Management
PBR Biodiversity and genetic variation
EPS
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) resistance genes - solanum-bulbocastanum - disease resistance - durable resistance - rxlr effectors - united-states - pathogen - population - races - diversity
Abstract Late blight is an important disease in potato that is caused by the oomycete Phytophthora infestans. In the past, Solanum demissum late blight resistance (R) genes were introgressed into cultivated potato (Solanum tuberosum). Eleven of these resistant plants were selected to characterize the virulence spectrum of individual P. infestans isolates and to monitor the dynamics of virulence in P. infestans populations. These plants are referred to as the Mastenbroek and Black differential sets. It has long been assumed that each differential plant contained one single R gene. In the current study and previous studies, however, most Mastenbroek differential plants were shown to harbor multiple R gene(s), which blurs virulence typing of late blight isolates. In order to acquire more accurate virulence profiles, we extended the Mastenbroek differential set with Solanum spp. plants harboring reduced R gene complexity and with plants containing recently identified R genes from related but different Solanum species. In addition, a differential set of ten Genetically Modified (GM) plants harboring single late blight R genes in the same genetic background (Desiree). By analyzing the virulence spectra of recently collected isolates using both newly described differential sets, we found that the GM Desiree differential set was more accurate for isolate virulence typing than the conventional (extended) differential set. Besides, the GM Desiree differential set was shown to be useful as trap plants to isolate novel P. infestans strains and to monitor virulence towards particular R genes in P. infestans populations `on site´. Legislative restrictions are, however, limiting the use of the GM Desiree differential set.
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