Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 486533
Title Effect of sublethal preculturing on the survival of probiotics and metabolite formation in set-yoghurt
Author(s) Settachaimongkon, S.; Valenberg, H.J.F. van; Winata, V.; Wang, X.; Nout, M.J.R.; Hooijdonk, A.C.M. van; Zwietering, M.H.; Smid, E.J.
Source Food Microbiology 49 (2015). - ISSN 0740-0020 - p. 104 - 115.
DOI https://doi.org/10.1016/j.fm.2015.01.011
Department(s) Food Quality and Design
Food Microbiology
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) lactic-acid bacteria - fermented milks - tolerance response - functional foods - stress responses - bifidobacteria - lactobacillus - cultures - strains - microorganisms
Abstract The objective of this study was to investigate the effect of preculturing of Lactobacillus rhamnosus GG and Bifidobacterium animalis subsp. lactis BB12 under sublethal stress conditions on their survival and metabolite formation in set-yoghurt. Prior to co-cultivation with yoghurt starters in milk, the two probiotic strains were precultured under sublethal stress conditions (combinations of elevated NaCl and low pH) in a batch fermentor. The activity of sublethally precultured probiotics was evaluated during fermentation and refrigerated storage by monitoring bacterial population dynamics, milk acidification and changes in volatile and non-volatile metabolite profiles of set-yoghurt. The results demonstrated adaptive stress responses of the two probiotic strains resulting in their viability improvement without adverse influence on milk acidification. A complementary metabolomic approach using SPME-GC/MS and 1H-NMR resulted in the identification of 35 volatiles and 43 non-volatile polar metabolites, respectively. Principal component analysis revealed substantial impact of the activity of sublethally precultured probiotics on metabolite formation demonstrated by distinctive volatile and non-volatile metabolite profiles of set-yoghurt. Changes in relative abundance of various aroma compounds suggest that incorporation of stress-adapted probiotics considerably influences the organoleptic quality of product. This study provides new information on the application of stress-adapted probiotics in an actual food-carrier environment
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