Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 488316
Title Comparative transcriptome analyses and genome assembly of Fusarium oxysporum f. sp. cubense
Author(s) Dita, M.A.; Herai, R.; Waalwijk, C.; Yamagishi, M.; Giachetto, P.; Ferreira, G.; Souza, M. de; Kema, G.H.J.
Source In: International ISHS-ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Use. - Leuven : ISHS - ISBN 9789066053595 - p. 165 - 168.
Event Leuven : ISHS - ISBN 9789066053595 International ISHS-ProMusa Symposium on Bananas and Plantains: Towards Sustainable Global Production and Improved Use, Salvador (Bahia), Brazil, 2011-10-10/2011-10-14
Department(s) Bioint Diagnostics, Food Safety & Phyt. Research
Entomology & Disease Management
Publication type Contribution in proceedings
Publication year 2013
Abstract Fusarium oxysporum f. sp. cubense (Foc), the causal agent of Fusarium wilt of banana, is a highly destructive and genetically diverse pathogen. Despite its economic importance, genomic information about Foc is limited and no transcriptomic analyses have been reported so far. By using 454 sequencing technology, we generated >2.5 million expressed sequenced tags (ESTs) from four Foc isolates representing different vegetative compatibility groups (VCGs) and races that infect banana: race 1 (R1, VCG unknown but different from the others here described), race 2 (R2, VCG 0124), subtropical race 4 (SR4, VCG 0120) and tropical race 4 (TR4, VCG 01213). The ESTs were obtained from libraries prepared from mRNA extracted from three physiological states (mycelium, conidia and germinated conidia), which were pooled in a 2:2:1 ratio. Most genes are represented in all libraries, but in silico comparative analyses identified a set of unique ESTs for each isolate (689 for R1, 974 for R2, 296 for SR4 and 555 for TR4), which are excellent candidates for diagnostics development, future plant-pathogen interaction studies and functional analyses. In subsequent analyses, a 40× sequencing-coverage (Illumina single reads) of TR4 genomic DNA was assembled in a de novo based methodology, resulting in a 45.5 Mb genome. Preliminary analyses show a high colinearity of EST and genomic data that significantly contributes to the quality of the assembly
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