Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 488721
Title Characterization of molecular mechanisms underlying the multi-drug-resistant phenotypes of Mycosphaerella graminicola field isolates
Author(s) Fillinger, S.; Omrane, S.; Sghyer, H.; Lanen, C.; Aoini, L.; Kema, G.H.J.
Source In: Modern fungicides and antifungal compounds VII. Proceedings of the 17th International Reinhardsbrunn Symposium. - Braunschweig : Deutsche Phytomedizinische Gesellschaft - ISBN 9783941261136 - p. 111 - 116.
Event Braunschweig : Deutsche Phytomedizinische Gesellschaft - ISBN 9783941261136 Proceedings of the 17th International Reinhardsbrunn Symposium, Friedrichroda, Germany, 2013-04-21/2013-04-25
Department(s) Entomology & Disease Management
Publication type Contribution in proceedings
Publication year 2013
Abstract Multidrug resistance (MDR) is a common trait developed by many organisms to counteract chemicals and/or drugs used against them. The basic MDR mechanism is relying on an overexpressed efflux transport system that actively expulses the toxic agent outside the cell. In fungi, MDR has been extensively studied in Saccharomyces cerevisiae and Candida albicans, but also plant pathogenic fungi are concerned by this phenomenon. In this study, we confirmed a drug efflux based resistance mechanism in two isolates of the septoria leaf blotch agent Mycosphaerella graminicola. Using next generation sequencing approaches, we could demonstrate that the MgMFS1 gene was strongly overexpressed in both isolates. The mutation(s) responsible for the MDR phenotype was mapped to a 38.5 kb genomic fragment on chromosome VII, covering also the MgMFS1 gene. Precise genetic mapping will then identify the mdr mutation(s) leading to MfMFS1 overexpressio Characterization of molecular mechanisms underlying the multi-drug-resistant phenotypes of Mycosphaerella graminicola field isolates. (PDF Download Available). Available from: http://www.researchgate.net/publication/271765795_Characterization_of_molecular_mechanisms_underlying_the_multi-drug-resistant_phenotypes_of_Mycosphaerella_graminicola_field_isolates [accessed Jun 15, 2015]
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.