Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 489012
Title R1-mediated immunity triggered by the Phytophthora infestans RXLR effector AVR1 is activated inside the nucleus
Author(s) Du, Y.; Bouwmeester, K.; Berg, J.; Govers, F.
Source In: Book of Abstracts Oomycete Molecular Genetics Network Meeting. - - p. 7 - 8.
Event Oomycete Molecular Genetics Network Meeting 2015, Pacific Grove, 2015-03-14/2015-03-17
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2015
Abstract Phytophthora infestans is a devastating plant pathogen that causes late blight on potato and tomato. To colonize host plants, P. infestans secretes effectors that can modulate host defence. Well-known are the RXLR effectors that are translocated into host cells to manipulate the cell machinery. To counteract the pathogen, potato exploits nucleotidebinding leucine-rich repeat (NLR) immune receptors that confer resistance against P. infestans upon recognition of a RXLR effector, with each NLR protein (or R protein) having its own matching RXLR effector (or AVR protein). The mechanisms underlying NLR-mediated resistance are still poorly understood. In this study we exploited fluorescent tags and nuclear localization and export signals (NLS/NES) for determining the subcellular localization of the potato NLR protein R1 and the P. infestans RXLR effector AVR1, and for targeting these proteins to nucleus or cytoplasm. Microscopic imaging revealed that both R1 and AVR1 occur in nucleus and cytoplasm, and in close proximity. Transient expression of NLS- or NES-tagged R1 and AVR1 in Nicotiana benthamiana showed that activation of R1-mediated hypersensitive response and resistance requires localization of the R1/AVR1 pair in the nucleus. However, AVR1-mediated suppression of cell death in absence of R1 is dependent on localization of AVR1 in the cytoplasm. A balanced nucleocytoplasmic partitioning of AVR1 seems to be a prerequisite.
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