Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 489603
Title A sensitive epitope-blocking ELISA for the detection of Chikungunya virus-specific antibodies in patients
Author(s) Goh, L.Y.H.; Kam, Y.W.; Metz, S.W.H.; Hobson-Peters, J.; Prow, N.A.; McCarthy, S.; Smith, D.W.; Pijlman, G.P.; Ng, L.F.P.; Hall, R.A.
Source Journal of Virological Methods 222 (2015). - ISSN 0166-0934 - p. 55 - 61.
DOI https://doi.org/10.1016/j.jviromet.2015.05.011
Department(s) Laboratory of Virology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) west-nile-virus - linked-immunosorbent-assay - valley encephalitis-virus - monoclonal-antibodies - diagnostic-accuracy - universal detection - reunion island - insect cells - ns1 protein - pcr assay
Abstract Chikungunya fever (CHIKF) has re-emerged as an arboviral disease that mimics clinical symptoms of other diseases such as dengue, malaria, as well as other alphavirus-related illnesses leading to problems with definitive diagnosis of the infection. Herein we describe the development and evaluation of a sensitive epitope-blocking ELISA (EB-ELISA) capable of specifically detecting anti-chikungunya virus (CHIKV) antibodies in clinical samples. The assay uses a monoclonal antibody (mAb) that binds an epitope on the E2 protein of CHIKV and does not exhibit cross-reactivity to other related alphaviruses. We also demonstrated the use of recombinant CHIK virus-like particles (VLPs) as a safe alternative antigen to infectious virions in the assay. Based on testing of 60 serum samples from patients in the acute or convalescent phase of CHIKV infection, the EB-ELISA provided us with 100% sensitivity, and exhibited 98.5% specificity when Ross River virus (RRV)- or Barmah Forest virus (BFV)-immune serum samples were included. This assay meets the public health demands of a rapid, robust, sensitive and specific, yet simple assay for specifically diagnosing CHIK-infections in humans.
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