Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 490022
Title Cultivation-Independent Screening Revealed Hot Spots of IncP-1, IncP-7 and IncP-9 Plasmid Occurrence in Different Environmental Habitats
Author(s) Dealtry, S.; Ding, G.C.; Weichelt, V.; Dunon, V.; Schluter, A.; Martini, M.C.; Papa, M.F. Del; Lagares, A.; Amos, G.C.A.; Wellington, E.M.H.; Gaze, W.H.; Sipkema, D.; Sjoling, S.; Springael, D.; Heuer, H.; Elsas, J.D.; Thomas, C.; Smalla, K.
Source PLoS ONE 9 (2014)2. - ISSN 1932-6203
DOI https://doi.org/10.1371/journal.pone.0089922
Department(s) Microbiology
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) resistance genes - naphthalene - pseudomonas - adaptation - prevalence - transposon - diversity - sediment - biobeds
Abstract IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC- DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are "hot spots'' of plasmids potentially carrying catabolic genes.
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