Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 490375
Title Impact of growth conditions and role of sigB on Listeria monocytogenes fitness in single and mixed biofilms cultured with Lactobacillus plantarum
Author(s) Saa Ibusquiza, P.; Nierop Groot, M.N.; Deban Valles, A.; Abee, T.; Besten, H.M.W. den
Source Food Research International 71 (2015). - ISSN 0963-9969 - p. 140 - 145.
Department(s) Food Microbiology
Food Technology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) lactic-acid bacteria - oxidative stress resistance - gram-positive bacteria - superoxide-dismutase - tolerance response - arginine deiminase - species biofilms - stainless-steel - sodium-chloride - sigma(b)
Abstract The role of sigB, a major transcriptional regulator of stress response genes, was assessed in formation of single and mixed species biofilms of Listeria monocytogenes EGD-e and Lactobacillus plantarum WCFS1 as secondary species at 20 °C and 30 °C using different medium compositions (nutrient-rich medium with and without supplementary manganese, glucose and salt). L. monocytogenes showed significant biofilm development at both temperatures and in all media tested although less biofilm was formed when glucose was supplemented only. The contribution of L. monocytogenes to the mixed species biofilm declined especially at higher temperature in glucose-rich medium in the absence and presence of manganese, due to lactic acid formation with concomitant decrease in culture pH below the pHmin of L. monocytogenes. Using an in-frame sigB deletion mutant and a complementation mutant we showed that sigB contributed to survival under these acid stress conditions. Notably, the additional presence of salt protected L. monocytogenes in the acidic mixed species biofilms resulting in an increase of around 2–3 log10 cfu/ml and this phenomenon showed to be sigB-dependent.
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