Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 490499
Title Fluorescent Lectins for Local in Vivo Visualization of Peripheral Nerves
Author(s) KleinJan, G.H.; Buckle, T.; Willigen, D.M. van; Oosterom, M.N. van; Spa, S.J.; Kloosterboer, H.E.; Leeuwen, F.W.B. van
Source Molecules 19 (2014)7. - ISSN 1420-3049 - p. 9876 - 9892.
Department(s) BioNanoTechnology
Publication type Refereed Article in a scientific journal
Publication year 2014
Keyword(s) nervous-system - sentinel node - immunohistochemical localization - radical prostatectomy - sulfate proteoglycan - axonal-transport - guided surgery - identification - carcinoma - binding
Abstract Damage to peripheral nerves caused during a surgical intervention often results in function loss. Fluorescence imaging has the potential to improve intraoperative identification and preservation of these structures. However, only very few nerve targeting agents are available. This study describes the in vivo nerve staining capabilities of locally administered fluorescent lectin-analogues. To this end WGA, PNA, PHA-L and LEL were functionalized with Cy5 (lambda(ex) (max) 640 nm; lambda(em) (max) 680 nm). Transfer of these imaging agents along the sciatic nerve was evaluated in Thy1-YFP mice (n = 12) after intramuscular injection. Migration from the injection site was assessed in vivo using a laboratory fluorescence scanner and ex vivo via fluorescence confocal microscopy. All four lectins showed retrograde movement and staining of the epineurium with a signal-to-muscle ratio of around two. On average, the longest transfer distance was obtained with WGA-Cy5 (0.95 cm). Since WGA also gave minimal uptake in the lymphatic system, this lectin type revealed the highest potential as a migration imaging agent to visualize nerves.
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