Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 490928
Title Ligation-Independent Cloning for Plant Research
Author(s) Wendrich, J.R.; Liao, C.Y.; Berg, W.A.M. van den; Rybel, B. De; Weijers, D.
Source In: Plant Functional Genomics : Methods and Protocols. / Alonso, J.M., Stepanova, A.N., [S.l.] : Springer (Methods in Molecular Biology 1284) - ISBN 9781493924431 - p. 421 - 431.
Department(s) Biochemistry
Publication type Peer reviewed book chapter
Publication year 2015
Abstract Molecular cloning is a vital step in much of today’s plant biological research. Particularly, when a species is amenable to transgenic manipulation, cloning enables detailed study of gene and protein function in vivo. Therefore, accurate, consistent, and efficient cloning methods have the potential to accelerate biological research. Traditional restriction-enzyme/ligase-based strategies are often inefficient, while novel alternative methods can be less economical. We have recently optimized a method for Ligation-Independent Cloning (LIC) that is both efficient and economical. We have developed a large set of LIC-compatible plasmids for application in plant research. These include dedicated vectors for gene expression analysis, protein localization studies, and protein misexpression. We describe a detailed protocol that allows the reliable generation of plant transformation-ready constructs from PCR fragments in 2–3 days.
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