Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 491031
Title Immune-relevant thrombocytes of common carp undergo parasite-induced nitric oxide-mediated apoptosis
Author(s) Fink, I.R.; Ribeiro, C.M.S.; Forlenza, M.; Taverne-Thiele, J.J.; Rombout, J.H.W.M.; Savelkoul, H.F.J.; Wiegertjes, G.
Source Developmental and Comparative Immunology 50 (2015)2. - ISSN 0145-305X - p. 146 - 154.
DOI https://doi.org/10.1016/j.dci.2015.02.008
Department(s) Cell Biology and Immunology
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) lymphocyte maturation factor - cyprinus-carpio - expression analysis - human platelets - functional-characterization - monoclonal-antibodies - cell-differentiation - teleost fish - genes - l.
Abstract Common carp thrombocytes account for 30–40% of peripheral blood leukocytes and are abundant in the healthy animals' spleen, the thrombopoietic organ. We show that, ex vivo, thrombocytes from healthy carp express a large number of immune-relevant genes, among which several cytokines and Toll-like receptors, clearly pointing at immune functions of carp thrombocytes. Few studies have described the role of fish thrombocytes during infection. Carp are natural host to two different but related protozoan parasites, Trypanoplasma borreli and Trypanosoma carassii, which reside in the blood and tissue fluids. We used the two parasites to undertake controlled studies on the role of fish thrombocytes during these infections. In vivo, but only during infection with T. borreli, thrombocytes were massively depleted from the blood and spleen leading to severe thrombocytopenia. Ex vivo, addition of nitric oxide induced a clear and rapid apoptosis of thrombocytes from healthy carp, supporting a role for nitric oxide-mediated control of immune-relevant thrombocytes during infection with T. borreli. The potential advantage for parasites to selectively deplete the host of thrombocytes via nitric oxide-induced apoptosis is discussed.
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