Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 492858
Title Influence of protein and carbohydrate contents of soy protein hydrolysates on cell density and IgG production in animal cell cultures
Author(s) Gupta, A.J.; Wierenga, P.A.; Gruppen, H.; Boots, J.W.
Source Biotechnology Progress 31 (2015)5. - ISSN 8756-7938 - p. 1396 - 1405.
DOI https://doi.org/10.1002/btpr.2121
Department(s) Food Chemistry
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract The variety of compounds present in chemically defined media as well as media supplements makes it difficult to use a mechanistic approach to study the effect of supplement composition on culture functionality. Typical supplements, such as soy protein hydrolysates contain peptides, amino acids, carbohydrates, isoflavones, and saponins. To study the relative contribution of these compound classes, a set of hydrolysates were produced, containing 58-83% proteinaceous material and 5-21% carbohydrates. While the content of the different compounds classes varied, the composition (e.g., peptide profiles, carbohydrate composition) did not vary in hydrolysates. The hydrolysates were supplemented to a chemically defined medium in cell culture, based on equal weight and on equal protein levels. The latter showed that an increase in the carbohydrate concentration significantly (P value¿
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