Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 492874
Title Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes
Author(s) Künne, T.A.; Westra, E.R.; Brouns, S.J.J.
Source In: CRISPR : Methods and Protocols / Lundgren, M., Charpentier, E., Fineran, P.C., Springer Humana Press (Methods Molecular Biology 1311) - ISBN 9781493926862 - p. 171 - 184.
Department(s) Microbiology
Publication type Peer reviewed book chapter
Publication year 2015
Abstract The Electrophoretic Mobility Shift Assay is a straightforward and inexpensive method for the determination and quantification of protein-nucleic acid interactions. It relies on the different mobility of free and protein-bound nucleic acid in a gel matrix during electrophoresis. Nucleic acid affinities of crRNA-Cas complexes can be quantified by calculating the dissociation constant (Kd). Here, we describe how two types of EMSA assays are performed using the Cascade ribonucleoprotein complex from Escherichia coli as an example
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