Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 494022
Title Flow-Through Microbial Capture by Antibody-Coated Microsieves
Author(s) Nguyen, A.T.; Doorn, R. van; Baggerman, J.; Paulusse, J.M.J.; Klerks, M.M.; Zuilhof, H.; Rijn, C.J.M. van
Source Advanced Material Interfaces 2 (2015)3. - ISSN 2196-7350 - 9 p.
DOI https://doi.org/10.1002/admi.201400292
Department(s) Organic Chemistry
Biointeracties and Plant Health
Biological Farming Systems
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2015
Abstract A new flow-through method for rapid capture and detection of microorganisms is developed using optically-flat microengineered membranes. Selective and efficient capture of Salmonella is demonstrated with antibodies coated on membranes (microsieves) having a pore size much larger than the microorganism itself. The silicon-nitride membranes are first photochemically coated with 1,2-epoxy-9-decene yielding stable Si–C and N–C linkages. The resultant epoxide-terminated microsieves are subsequently biofunctionalized with anti-Salmonella antibodies. The capture efficiency of antibody-coated microsieves with different pore sizes (2.0–5.0 µm) is studied with Salmonella enterica enterica serotype Typhimurium suspensions (107 cfu mL–1). The antibody-coated microsieves capture 52% (2 µm microsieves), 30% (3.5 µm microsieves), and 12% (5 µm microsieves) of Salmonella from the suspension. The influence of flow rate (0.8–16 µL min–1 mm–2) on the capture efficiency of antibody-coated 3.5 µm microsieves is investigated. The capture efficiency increases from ˜30% to ˜70% when the flow-rate decreases from 16 to 0.8 µL min–1 mm–2. Antibody-coated 3.5 µm microsieves can capture Salmonella rapidly and directly from fresh milk suspension (capture 35% at concentration of 80 cfu mL–1). The use of antibody-coated microsieves as microbial selective capture devices is thus shown to be highly promising for the direct capture of microorganisms.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.