Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 501590
Title DL-propargylglycine reduces blood pressure and renal injury but increases kidney weight in angiotensin-II infused rats
Author(s) Oosterhuis, Nynke R.; Frenay, Anne Roos S.; Wesseling, Sebas; Snijder, Pauline M.; Slaats, Gisela G.; Yazdani, Saleh; Fernandez, Bernadette O.; Feelisch, Martin; Giles, Rachel H.; Verhaar, Marianne C.; Joles, Jaap A.; Goor, Harry Van
Source Nitric oxide : biology and chemistry 49 (2015). - ISSN 1089-8603 - p. 56 - 66.
DOI https://doi.org/10.1016/j.niox.2015.07.001
Department(s) Toxicology
Publication type Refereed Article in a scientific journal
Publication year 2015
Keyword(s) Angiotensin-II - DL-Propargylglycine - Hydrogen sulfide - Hypertension - Kidney weight - Proteinuria
Abstract

Hydrogen sulfide (H2S), carbon monoxide (CO) and nitric oxide (NO) share signaling and vasorelaxant properties and are involved in proliferation and apoptosis. Inhibiting NO production or availability induces hypertension and proteinuria, which is prevented by concomitant blockade of the H2S producing enzyme cystathionine γ-lyase (CSE) by d,l-propargylglycine (PAG). We hypothesized that blocking H2S production ameliorates Angiotensin II (AngII)-induced hypertension and renal injury in a rodent model. Effects of concomitant administration of PAG or saline were therefore studied in healthy (CON) and AngII hypertensive rats. In CON rats, PAG did not affect systolic blood pressure (SBP), but slightly increased proteinuria. In AngII rats PAG reduced SBP, proteinuria and plasma creatinine (180 ± 12 vs. 211 ± 19 mmHg; 66 ± 35 vs. 346 ± 92 mg/24 h; 24 ± 6 vs. 47 ± 15 μmol/L, respectively; p <0.01). Unexpectedly, kidney to body weight ratio was increased in all groups by PAG (p <0.05). Renal injury induced by AngII was reduced by PAG (p <0.001). HO-1 gene expression was increased by PAG alone (p <0.05). PAG increased inner cortical tubular cell proliferation after 1 week and decreased outer cortical tubular nucleus number/field after 4 weeks. In vitro proximal tubular cell size increased after exposure to PAG. In summary, blocking H2S production with PAG reduced SBP and renal injury in AngII infused rats. Independent of the cardiovascular and renal effects, PAG increased HO-1 gene expression and kidney weight. PAG alone increased tubular cell size and proliferation in-vivo and in-vitro. Our results are indicative of a complex interplay of gasotransmitter signaling/action of mutually compensatory nature in the kidney.

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