Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 503437
Title Induction of viable 2n pollen in sterile Oriental × Trumpet Lilium hybrids
Author(s) Luo, J.R.; Arens, P.; Niu, L.X.; Tuyl, J.M. van
Source Journal of Horticultural Science and Biotechnology 91 (2016)3. - ISSN 1462-0316 - p. 258 - 263.
Department(s) PBR Ornamentals, tissue culture and gene transfer
Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2016
Keyword(s) Interspecific hybridization - Laughing gas - Meiosis - OThybrids - Unreduced gametes

In order to induce viable 2n pollen from highly sterile diploid Oriental × Trumpet (OT) (Lilium), N2O was used to treat flower buds of four sterile diploid OT cultivars (‘Nymph’, ‘Gluhwein’, ‘Yelloween’, and ‘Shocking’) at different stages of meiosis. There was no pollen germination in the controls. However, after N2O treatment at 600 kPa, three of the OT hybrids (‘Nymph’, ‘Gluhwein’, and ‘Yelloween’) not only exhibited fertile flower percentages (4.3-16%), but also higher rates of pollen germination (18.8-72.5%). In addition, both the fertile flower percentages and rates of pollen germination were higher following 48 h N2O treatment at 600 kPa than the 24 h treatment. Following 72 h N2O treatment at 600 kPa, most flower buds or plants were damaged and any undamaged flowers showed abnormal anthers at the time of flowering. This indicated that 48 h of N2O treatment at 600 kPa was optimal to induce viable 2n pollen in three out of four sterile hybrid OT lily cultivars. These results also showed that prophase I - metaphase I was the optimum stage of meiosis at which to induce 2n pollen by N2O treatment in lily hybrids.

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