Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 515877
Title β2→1-fructans modulate the immune system in vivo in a microbiota-dependent and -independent fashion
Author(s) Fransen, Floris; Sahasrabudhe, Neha M.; Elderman, Marlies; Bosveld, Margaret; Aidy, Sahar El; Hugenholtz, Floor; Borghuis, Theo; Kousemaker, Ben; Winkel, Simon; Gaast-de Jongh, Christa van der; Jonge, Marien I. de; Boekschoten, Mark V.; Smidt, Hauke; Schols, Henk A.; Vos, Paul de
Source Frontiers in Immunology 8 (2017). - ISSN 1664-3224
DOI https://doi.org/10.3389/fimmu.2017.00154
Department(s) Food Chemistry
Laboratory of Plant Physiology
Microbiology
Nutrition, Metabolism and Genomics
VLAG
WIMEK
Publication type Refereed Article in a scientific journal
Publication year 2017
Keyword(s) Germ-free mice - Gut microbiota - Mucosal immunology - Prebiotics - β2→1-fructans
Abstract It has been shown in vitro that only specific dietary fibers contribute to immunity, but studies in vivo are not conclusive. Here, we investigated degree of polymerization (DP) dependent effects of β2→1-fructans on immunity via microbiota-dependent and -independent effects. To this end, conventional or germ-free mice received short- or long-chain β2→1-fructan for 5 days. Immune cell populations in the spleen, mesenteric lymph nodes (MLNs), and Peyer's patches (PPs) were analyzed with flow cytometry, genome-wide gene expression in the ileum was measured with microarray, and gut microbiota composition was analyzed with 16S rRNA sequencing of fecal samples. We found that β2→1-fructans modulated immunity by both microbiota and microbiota-independent effects. Moreover, effects were dependent on the chain-length of the β2→1-fructans type polymer. Both short- and long-chain β2→1-fructans enhanced T-helper 1 cells in PPs, whereas only short-chain β2→1-fructans increased regulatory T cells and CD11b-CD103- dendritic cells (DCs) in the MLN. A common feature after short- and long-chain β2→1-fructan treatment was enhanced 2-alpha-l-fucosyltransferase 2 expression and other IL-22-dependent genes in the ileum of conventional mice. These effects were not associated with shifts in gut microbiota composition, or altered production of short-chain fatty acids. Both short- and long-chain β2→1-fructans also induced immune effects in germ-free animals, demonstrating direct effect independent from the gut microbiota. Also, these effects were dependent on the chain-length of the β2→1-fructans. Short-chain β2→1-fructan induced lower CD80 expression by CD11b-CD103- DCs in PPs, whereas long-chain β2→1-fructan specifically modulated B cell responses in germ-free mice. In conclusion, support of immunity is determined by the chemical structure of β2→1-fructans and is partially microbiota independent.
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