Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 538963
Title A constraint-based model analysis of enterocyte mitochondrial adaptation to dietary interventions of lipid type and lipid load
Author(s) Sinha, Neeraj; Suarez-Diez, Maria; Hooiveld, Guido J.E.J.; Keijer, Jaap; Santos, Vitor Martin dos; Schothorst, Evert M. van
Source Frontiers in Physiology 9 (2018). - ISSN 1664-042X
Department(s) Human Nutrition & Health
Systems and Synthetic Biology
Nutrition, Metabolism and Genomics
Human and Animal Physiology
Publication type Refereed Article in a scientific journal
Publication year 2018
Keyword(s) Constraint-based metabolic model - Enterocytes - High fat diet - Mitochondria - Mitochondrial dynamics - Omega-3 lipids

Computational modeling of mitochondrial adaptability and flexibility in the small intestine upon different nutritional exposures will provide insights that will help to define healthy diet interventions. Therefore, a murine enterocyte-specific mitochondrial constraint-based metabolic model (named MT_mmuENT127) was constructed and used to simulate mitochondrial behavior under different dietary conditions, representing various levels and composition of nutrients absorbed by the enterocytes in mice, primarily focusing on metabolic pathways. Our simulations predicted that increasing the fraction of marine fatty acids in the diet, or increasing the dietary lipid/carbohydrate ratio resulted in (i) an increase in mitochondrial fatty acid beta oxidation, and (ii) changes in only a limited subset of mitochondrial reactions, which appeared to be independent of gene expression regulation. Moreover, transcript levels of mitochondrial proteins suggested unaltered fusion-fission dynamics by an increased lipid/carbohydrates ratio or by increased fractions of marine fatty acids. In conclusion, our enterocytic mitochondrial constraint-based model was shown to be a suitable platform to investigate effects of dietary interventions on mitochondrial adaptation and provided novel and deeper insights in mitochondrial metabolism and regulation.

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