Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 539500
Title Influence of substrate concentration on the extent of protein enzymatic hydrolysis
Author(s) Deng, Yuxi; Butré, Claire I.; Wierenga, P.A.
Source International Dairy Journal 86 (2018). - ISSN 0958-6946 - p. 39 - 48.
Department(s) Food Chemistry
Publication type Refereed Article in a scientific journal
Publication year 2018

Changes in substrate concentration were shown to affect the experimental maximum degree of hydrolysis (DHmax,exp). Such changes may affect the outcome of in vitro protein digestion models. To study this effect, apo α-lactalbumin (0.5–10%) was hydrolysed by digestive enzymes, bovine, porcine and human trypsins, bovine α-chymotrypsin or Bacillus licheniformis protease (BLP), at a constant enzyme to substrate ratio (E:S). Hydrolysis by human trypsin was not sensitive to changes in substrate concentration. The DHmax,exp by bovine and porcine trypsins, or bovine α-chymotrypsin increased with an increasing substrate concentration, while the DHmax,exp by BLP decreased. For hydrolyses with different DHmax,exp, a change in the percentage of intact protein versus DH was observed, meaning that the peptide concentrations differed during the hydrolyses. If some peptides could be protease inhibitors, the differences in DHmax,exp could be explained. This was confirmed by performing the hydrolysis at a higher E:S, which reached a higher DHmax,exp.

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