Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 542365
Title The effect of DNA extraction methods on observed microbial communities from fibrous and liquid rumen fractions of dairy cows
Author(s) Vaidya, J.D.; Bogert, B. van den; Edwards, J.E.; Boekhorst, Jos; Gastelen, S. van; Saccenti, E.; Plugge, C.M.; Smidt, H.
Department(s) Microbiology
VLAG
Animal Nutrition
Systems and Synthetic Biology
WIMEK
Publication type Dataset
Publication year 2017
Keyword(s) PRJEB22996 - ERP104729
Abstract DNA based methods have been widely used to study the complexity of the rumen microbiota, and it is well known that the method of DNA extraction is a critical step in enabling accurate assessment of this complexity. Rumen fluid (RF) and fibrous content (FC) fractions differ substantially in terms of their physical nature and associated microorganisms. The aim of this study was therefore to assess the effect of four DNA extraction methods (RBB, PBB, FDSS, PQIAmini) on the observed microbial diversity in RF and FC fractions using samples from four rumen cannulated dairy cows fed four diets with different ratios of grass and maize silage.
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.