Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 544272
Title Diacylglycerol acyltransferase 1 contributes to freezing tolerance
Author(s) Arisz, Steven A.; Heo, Jae-Yun; Koevoets, Iko Tamar; Zhao, Tao; Egmond, Pieter van; Meyer, Jessica; Zeng, Weiqing; Niu, Xiaomu; Wang, Baosheng; Mitchell-Olds, Thomas; Schranz, M.E.; Testerink, Christa
Source Plant Physiology 177 (2018)4. - ISSN 0032-0889 - p. 1410 - 1424.
DOI https://doi.org/10.1104/pp.18.00503
Department(s) Laboratory of Plant Physiology
Biosystematics
EPS
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract Freezing limits plant growth and crop productivity, and plant species in temperate zones have the capacity to develop freezing tolerance through complex modulation of gene expression affecting various aspects of metabolism and physiology. While many components of freezing tolerance have been identified in model species under controlled laboratory conditions, little is known about the mechanisms that impart freezing tolerance in natural populations of wild species. Here, we performed a quantitative trait locus (QTL) study of acclimated freezing tolerance in seedlings of Boechera stricta, a highly adapted relative of Arabidopsis thaliana native to the Rocky Mountains. A single QTL was identified that contained the gene encoding ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE 1 (BstDGAT1), whose expression is highly cold responsive. The primary metabolic enzyme DGAT1 catalyzes the final step in assembly of triacylglycerol (TAG) by acyl transfer from acyl-CoA to diacylglycerol. Freezing tolerant plants showed higher DGAT1 expression during cold acclimation than more sensitive plants and this resulted in increased accumulation of TAG in response to subsequent freezing. Levels of oligogalactolipids which are produced by SFR2 (SENSITIVE TO FREEZING 2), an indispensable element of freezing tolerance in Arabidopsis, were also higher in freezing tolerant plants. Furthermore, overexpression of AtDGAT1 led to increased freezing tolerance. We propose that DGAT1 confers freezing tolerance in plants by supporting SFR2-mediated remodeling of chloroplast membranes.
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