Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 544899
Title Mechanistic study on trophic interaction between mucosal keystone species and butyrogenic gut commensals
Author(s) Chia, L.W.; Hornung, B.V.H.; Aalvink, S.; Schaap, P.J.; Vos, W.M. de; Knol, J.; Belzer, C.
Department(s) Microbiology
Systems and Synthetic Biology
Publication type Dataset
Publication year 2018
Keyword(s) PRJEB20031 - ERP022138 - Akkermansia muciniphilaAkkermansia muciniphila
Abstract Host glycans are paramount in regulating the symbiotic relationship between humans and their gut bacteria. The constant flux of host-secreted mucin at the mucosal layer creates a steady niche space for bacteria colonization. Mucin, characterized by complex molecular structure, exerts selective nutritional pressure for mucin-degrading bacteria. Mucin degradation by keystone species subsequently drives the local trophic chain and shapes mucosal microbial assembly a.k.a. mucobiome. This study investigates mucin-driven trophic interaction between the specialized mucin-degrader, Akkermansia muciniphila and butyrogenic gut commensals. Co-cultures of A. muciniphila with non-mucolytic butyrogens (Anaerostipes caccae and Eubacterium hallii) were grown in minimal media supplemented with pure mucin. Metabolites (HPLC) and meta-transcriptome (RNA-seq) were studied. Mucin degradation by A. muciniphila produced mucin-derived monosaccharides and metabolites (galactose, fucose, mannose, GlcNAc and acetate) for the growth of butyrogens (A. caccae and E. hallii) resulted in 2mM butyrate production. Interestingly, co-culture of A. muciniphila with E. hallii demonstrated mutual relationship, in which pseudovitamin B12 production by E. hallii facilitated propionate production by A. muciniphila. Cobalamin-dependent methylmalonyl-CoA mutase genes (Amuc_1983 and Amuc_1984) were upregulated in A. muciniphila monoculture, indicated the attempt by A. muciniphila to activate propionate production pathway by synthesizing more key catalytic enzymes. Differential analysis (DESeq2) showed the presence of butyrogens resulted in an altered transcriptional profile of A. muciniphila. E. hallii in particular, incurred high functional impact on A. muciniphila gene expression. Mucosal subpopulation driven by A. muciniphila could result in butyrate and propionate production. Deciphering the underlying mechanism of this microbial tropism is crucial for the understanding of mucosal health and pathophysiology.
There are no comments yet. You can post the first one!
Post a comment
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.