|Title||Renaissance of traditional DNA transfer strategies for improvement of industrial lactic acid bacteria|
|Author(s)||Bron, Peter A.; Marcelli, Barbara; Mulder, Joyce; Els, Simon van der; Morawska, Luiza P.; Kuipers, Oscar P.; Kok, Jan; Kleerebezem, Michiel|
|Source||Current Opinion in Biotechnology 56 (2019). - ISSN 0958-1669 - p. 61 - 68.|
|Publication type||Refereed Article in a scientific journal|
The ever-expanding genomic insight in natural diversity of lactic acid bacteria (LAB) has revived the industrial interest in traditional and natural genetic mobilization methodologies. Here, we review recent advances in horizontal gene transfer processes in LAB, including natural competence, conjugation, and phage transduction. In addition, we envision the possibilities for industrial strain improvement arising from the recent discoveries of molecular exchanges between bacteria through nanotubes and extracellular vesicles, as well as the constantly expanding genome editing possibilities using the CRISPR-Cas technology.