|Title||Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats|
|Author(s)||Baliu-Piqué, Mariona; Kurniawan, Henry; Ravesloot, Lars; Verheij, Myrddin W.; Drylewicz, Julia; Lievaart-Peterson, Karianne; Borghans, José A.M.; Koets, Ad; Tesselaar, Kiki|
|Source||Developmental and Comparative Immunology 93 (2019). - ISSN 0145-305X - p. 1 - 10.|
|Department(s)||Bacteriology & Epidemiology|
|Publication type||Refereed Article in a scientific journal|
|Keyword(s)||Deuterium - Development - Goat - Mathematical modelling - Neonatal adaptive immunity - Stable isotope labelling - T-cell turnover - T-lymphocytes|
Neonatal mammals have increased disease susceptibility and sub-optimal vaccine responses. This raises problems in both humans and farm animals. The high prevalence of paratuberculosis in goats and the lack of an effective vaccine against it have a strong impact on the dairy sector, and calls for vaccines optimized for the neonatal immune system. We characterized the composition of the T-cell pool in neonatal kids and adult goats and quantified their turnover rates using in vivo deuterium labelling. From birth to adulthood, CD4+ T-cells were the predominant subset in the thymus and lymph nodes, while spleen and bone marrow contained mainly CD8+ lymphocytes. In blood, CD4+ T-cells were the predominant subset during the neonatal period, while CD8+ T-cells predominated in adults. We observed that thymic mass and cellularity increased during the first 5 months after birth, but decreased later in life. Deuterium labelling revealed that T-cell turnover rates in neonatal kids are considerably higher than in adult animals.