Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 545607
Title Diet-independent correlations between bacteria and dysfunction of gut, adipose tissue, and liver : A comprehensive microbiota analysis in feces and mucosa of the ileum and colon in obese mice with NAFLD
Author(s) Gart, Eveline; Lima, Everton Souto; Schuren, Frank; Ruiter, Christa G.F. de; Attema, Joline; Verschuren, Lars; Keijer, Jaap; Salic, Kanita; Morrison, Martine C.; Kleemann, Robert
Source International Journal of Molecular Sciences 20 (2019)1. - ISSN 1661-6596
DOI https://doi.org/10.3390/ijms20010001
Department(s) Human and Animal Physiology
WIAS
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Adipose tissue inflammation - Gut permeability - Liver - Non-alcoholic fatty liver disease - Obesity - Short-chain fatty acids
Abstract

Development of non-alcoholic fatty liver disease (NAFLD) is linked to obesity, adipose tissue inflammation, and gut dysfunction, all of which depend on diet. So far, studies have mainly focused on diet-related fecal microbiota changes, but other compartments may be more informative on host health. We present a first systematic analysis of microbiota changes in the ileum and colon using multiple diets and investigating both fecal and mucosal samples. Ldlr−/−.Leiden mice received one of three different energy-dense (ED)-diets (n = 15/group) for 15 weeks. All of the ED diets induced obesity and metabolic risk factors, altered short-chain fatty acids (SCFA), and increased gut permeability and NAFLD to various extents. ED diets reduced the diversity of high-abundant bacteria and increased the diversity of low-abundant bacteria in all of the gut compartments. The ED groups showed highly variable, partially overlapping microbiota compositions that differed significantly from chow. Correlation analyses demonstrated that (1) specific groups of bacteria correlate with metabolic risk factors, organ dysfunction, and NAFLD endpoints, (2) colon mucosa had greater predictive value than other compartments, (3) correlating bacteria differed per compartment, and (4) some bacteria correlated with plasma SCFA levels. In conclusion, this comprehensive microbiota analysis demonstrates correlations between the microbiota and dysfunctions of gut, adipose tissue, and liver, independent of a specific disease-inducing diet.

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