Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 545745
Title Phytophthora infestans RXLR effectors act in concert at diverse subcellular locations to enhance host colonization
Author(s) Wang, Shumei; McLellan, Hazel; Bukharova, Tatyana; He, Qin; Murphy, Fraser; Shi, Jiayang; Sun, Shaohui; Weymers, Pauline van; Ren, Yajuan; Thilliez, Gaetan; Wang, Haixia; Chen, Xinwei; Engelhardt, Stefan; Vleeshouwers, Vivianne; Gilroy, Eleanor M.; Whisson, Stephen C.; Hein, Ingo; Wang, Xiaodan; Tian, Zhendong; Birch, Paul R.J.; Boevink, Petra C.
Source Journal of Experimental Botany 70 (2019)1. - ISSN 0022-0957 - p. 343 - 356.
Department(s) Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2019

Oomycetes such as the potato blight pathogen Phytophthora infestans deliver RXLR effectors into plant cells to manipulate host processes and promote disease. Knowledge of where they localize inside host cells is important in understanding their function. Fifty-two P. infestans RXLR effectors (PiRXLRs) up-regulated during early stages of infection were expressed as fluorescent protein (FP) fusions inside cells of the model host Nicotiana benthamiana. FP-PiRXLR fusions were predominantly nucleo-cytoplasmic, nuclear, or plasma membrane-associated. Some also localized to the endoplasmic reticulum, mitochondria, peroxisomes, or microtubules, suggesting diverse sites of subcellular activity. Seven of the 25 PiRXLRs examined during infection accumulated at sites of haustorium penetration, probably due to co-localization with host target processes; Pi16663 (Avr1), for example, localized to Sec5-associated mobile bodies which showed perihaustorial accumulation. Forty-five FP-RXLR fusions enhanced pathogen leaf colonization when expressed in Nicotiana benthamiana, revealing that their presence was beneficial to infection. Co-expression of PiRXLRs that target and suppress different immune pathways resulted in an additive enhancement of colonization, indicating the potential to study effector combinations using transient expression assays. We provide a broad platform of high confidence P. infestans effector candidates from which to investigate the mechanisms, singly and in combination, by which this pathogen causes disease.

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