Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 546036
Title Modulating D-amino acid oxidase (DAAO) substrate specificity through facilitated solvent access
Author(s) Subramanian, Kalyanasundaram; Góra, Artur; Spruijt, Ruud; Mitusińska, Karolina; Suarez-Diez, Maria; Martins Dos Santos, Vitor; Schaap, Peter J.
Source PLoS ONE 13 (2018)6. - ISSN 1932-6203
DOI https://doi.org/10.1371/journal.pone.0198990
Department(s) Systems and Synthetic Biology
Biophysics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2018
Abstract

D-amino acid oxidase (DAAO) degrades D-amino acids to produce α-ketoacids, hydrogen peroxide and ammonia. DAAO has often been investigated and engineered for industrial and clinical applications. We combined information from literature with a detailed analysis of the structure to engineer mammalian DAAOs. The structural analysis was complemented with molecular dynamics simulations to characterize solvent accessibility and product release mechanisms. We identified non-obvious residues located on the loops on the border between the active site and the secondary binding pocket essential for pig and human DAAO substrate specificity and activity. We engineered DAAOs by mutating such critical residues and characterised the biochemical activity of the resulting variants. The results highlight the importance of the selected residues in modulating substrate specificity, product egress and enzyme activity, suggesting further steps of DAAO re-engineering towards desired clinical and industrial applications.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.