Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 546163
Title Epigenetic mapping of the Arabidopsis metabolome reveals mediators of the epigenotype-phenotype map
Author(s) Kooke, R.; Morgado, L.N.; Becker, F.F.M.; Eekelen, H.D.L.M. van; Hazarika, Rashmi; Zhang, Qunfeng; Vos, C.H. de; Johannes, Frank; Keurentjes, J.J.B.
Source Genome Research 29 (2019)1. - ISSN 1088-9051 - p. 96 - 106.
DOI https://doi.org/10.1101/gr.232371.117
Department(s) Mathematical and Statistical Methods - Biometris
Laboratory of Genetics
Biosystematics
BIOS Applied Metabolic Systems
EPS
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract Identifying the sources of natural variation underlying metabolic differences between plants will enable a better understanding of plant metabolism and provide insights into the regulatory networks that govern plant growth and morphology. So far, however, the contribution of epigenetic variation to metabolic diversity has been largely ignored. In the present study, we utilized a panel of Arabidopsis thaliana epigenetic recombinant inbred lines (epiRILs) to assess the impact of epigenetic variation on the metabolic composition. Thirty epigenetic QTL (QTLepi) were detected, which partly overlap with QTLepi linked to growth and morphology. In an effort to identify causal candidate genes in the QTLepi regions or their putative trans-targets we performed in silico small RNA and qPCR analyses. Differentially expressed genes were further studied by phenotypic and metabolic analyses of knockout mutants. Three genes were detected that recapitulated the detected QTLepi effects, providing evidence for epigenetic regulation in cis and in trans. These results indicate that epigenetic mechanisms impact metabolic diversity, possibly via small RNAs, and thus aid in further disentangling the complex epigenotype-phenotype map.
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