Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 546191
Title CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation
Author(s) Lindeboom, Jelmer J.; Nakamura, Masayoshi; Saltini, Marco; Hibbel, Anneke; Walia, Ankit; Ketelaar, Tijs; Emons, Anne Mie C.; Sedbrook, John C.; Kirik, Viktor; Mulder, Bela M.; Ehrhardt, David W.
Source Journal of Cell Biology 218 (2019)1. - ISSN 0021-9525 - p. 190 - 205.
Department(s) Laboratory of Cell Biology
Publication type Refereed Article in a scientific journal
Publication year 2019

Central to the building and reorganizing cytoskeletal arrays is creation of new polymers. Although nucleation has been the major focus of study for microtubule generation, severing has been proposed as an alternative mechanism to create new polymers, a mechanism recently shown to drive the reorientation of cortical arrays of higher plants in response to blue light perception. Severing produces new plus ends behind the stabilizing GTP-cap. An important and unanswered question is how these ends are stabilized in vivo to promote net microtubule generation. Here we identify the conserved protein CLASP as a potent stabilizer of new plus ends created by katanin severing in plant cells. Clasp mutants are defective in cortical array reorientation. In these mutants, both rescue of shrinking plus ends and the stabilization of plus ends immediately after severing are reduced. Computational modeling reveals that it is the specific stabilization of severed ends that best explains CLASP's function in promoting microtubule amplification by severing and array reorientation.

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