Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 546265
Title NADP-MALIC ENZYME 1 Affects Germination after Seed Storage in Arabidopsis thaliana
Author(s) Yazdanpanah, F.; Maurino, Veronica; Buijs, G.; Karimi Jashni, M.; Willems, L.A.J.; Sergeeva, L.; Rajjou, Loïc; Hilhorst, H.W.M.; Bentsink, L.
Source Plant and Cell Physiology 60 (2019)2. - ISSN 0032-0781 - p. 318 - 328.
DOI https://doi.org/10.1093/pcp/pcy213
Department(s) Laboratory of Plant Physiology
EPS
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract Aging decreases the quality of seeds and results in agricultural
and economic losses. The damage that occurs at the
biochemical level can alter the seed physiological status.
Although loss of viability has been investigated frequently,
little information exists on the molecular and biochemical
factors involved in seed deterioration and loss of viability.
Oxidative stress has been implicated as a major contributor
to seed deterioration, and several pathways are involved in
protection against this. In this study, we show that seeds of
Arabidopsis thaliana lacking a functional NADP-MALIC
ENZYME 1 (NADP-ME1) have reduced seed viability relative
to the wild type. Seeds of the NADP-ME1 loss-of-function
mutant display higher levels of protein carbonylation than
those of the wild type. NADP-ME1 catalyzes the oxidative
decarboxylation of malate to pyruvate with the simultaneous
production of CO2 and NADPH. Upon seed imbibition,
malate and amino acids accumulate in embryos of aged
seeds of the NADP-ME1 loss-of-function mutant compared
with those of the wild type. NADP-ME1 expression is
increased in imbibed aged as compared with non-aged
seeds. NADP-ME1 activity at testa rupture promotes
normal germination of aged seeds. In seedlings of aged
seeds, NADP-ME1 is specifically active in the root meristematic
zone. We propose that NADP-ME1 activity is required
for protecting seeds against oxidation during seed dry
storage.
Keywords: Ageing Arabidopsis thaliana Enzyme activity
Gene expression Meristematic zone Metabolite
profiling Oxidative stress Seed longevity.
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