Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 547357
Title Mobile PEAR transcription factors integrate positional cues to prime cambial growth
Author(s) Miyashima, Shunsuke; Roszak, Pawel; Sevilem, Iris; Toyokura, Koichi; Blob, Bernhard; Heo, Jung-Ok; Mellor, Nathan; Help-Rinta-Rahko, Hanna; Otero, Sofia; Smet, Wouter; Boekschoten, Mark; Hooiveld, Guido; Hashimoto, Kayo; Smetana, Ondřej; Siligato, Riccardo; Wallner, Eva Sophie; Mähönen, Ari Pekka; Kondo, Yuki; Melnyk, Charles W.; Greb, Thomas; Nakajima, Keiji; Sozzani, Rosangela; Bishopp, Anthony; Rybel, Bert de; Helariutta, Ykä
Source Nature 565 (2019)7740. - ISSN 0028-0836 - p. 490 - 494.
DOI https://doi.org/10.1038/s41586-018-0839-y
Department(s) EPS
Biochemistry
Nutrition, Metabolism and Genomics
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

Apical growth in plants initiates upon seed germination, whereas radial growth is primed only during early ontogenesis in procambium cells and activated later by the vascular cambium1. Although it is not known how radial growth is organized and regulated in plants, this system resembles the developmental competence observed in some animal systems, in which pre-existing patterns of developmental potential are established early on2,3. Here we show that in Arabidopsis the initiation of radial growth occurs around early protophloem-sieve-element cell files of the root procambial tissue. In this domain, cytokinin signalling promotes the expression of a pair of mobile transcription factors—PHLOEM EARLY DOF 1 (PEAR1) and PHLOEM EARLY DOF 2 (PEAR2)—and their four homologues (DOF6, TMO6, OBP2 and HCA2), which we collectively name PEAR proteins. The PEAR proteins form a short-range concentration gradient that peaks at protophloem sieve elements, and activates gene expression that promotes radial growth. The expression and function of PEAR proteins are antagonized by the HD-ZIP III proteins, well-known polarity transcription factors4—the expression of which is concentrated in the more-internal domain of radially non-dividing procambial cells by the function of auxin, and mobile miR165 and miR166 microRNAs. The PEAR proteins locally promote transcription of their inhibitory HD-ZIP III genes, and thereby establish a negative-feedback loop that forms a robust boundary that demarks the zone of cell division. Taken together, our data establish that during root procambial development there exists a network in which a module that links PEAR and HD-ZIP III transcription factors integrates spatial information of the hormonal domains and miRNA gradients to provide adjacent zones of dividing and more-quiescent cells, which forms a foundation for further radial growth.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.