Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 547982
Title Age-associated Impairment of the Mucus Barrier Function is Associated with Profound Changes in Microbiota and Immunity
Author(s) Sovran, Bruno; Hugenholtz, Floor; Elderman, Marlies; Beek, Adriaan A. Van; Graversen, Katrine; Huijskes, Myrte; Boekschoten, Mark V.; Savelkoul, Huub F.J.; Vos, Paul De; Dekker, Jan; Wells, Jerry M.
Source Scientific Reports 9 (2019)1. - ISSN 2045-2322
DOI https://doi.org/10.1038/s41598-018-35228-3
Department(s) Host-Microbe Interactomics
VLAG
Microbiology
Cell Biology and Immunology
Nutrition, Metabolism and Genomics
WIAS
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

Aging significantly increases the vulnerability to gastrointestinal (GI) disorders but there are few studies investigating the key factors in aging that affect the GI tract. To address this knowledge gap, we used 10-week- and 19-month-old litter-mate mice to investigate microbiota and host gene expression changes in association with ageing. In aged mice the thickness of the colonic mucus layer was reduced about 6-fold relative to young mice, and more easily penetrable by luminal bacteria. This was linked to increased apoptosis of goblet cells in the upper part of the crypts. The barrier function of the small intestinal mucus was also compromised and the microbiota were frequently observed in contact with the villus epithelium. Antimicrobial Paneth cell factors Ang4 and lysozyme were expressed in significantly reduced amounts. These barrier defects were accompanied by major changes in the faecal microbiota and significantly decreased abundance of Akkermansia muciniphila which is strongly and negatively affected by old age in humans. Transcriptomics revealed age-associated decreases in the expression of immunity and other genes in intestinal mucosal tissue, including decreased T cell-specific transcripts and T cell signalling pathways. The physiological and immunological changes we observed in the intestine in old age, could have major consequences beyond the gut.

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