|Title||Limited contribution of non-intensive chicken farming to ESBL-producing Escherichia coli colonization in humans in Vietnam : an epidemiological and genomic analysis|
|Author(s)||Nguyen, Vinh Trung; Jamrozy, Dorota; Matamoros, Sébastien; Carrique-Mas, Juan J.; Ho, Huynh Mai; Thai, Quoc Hieu; Nguyen, Thi Nhu Mai; Wagenaar, Jaap A.; Thwaites, Guy; Parkhill, Julian; Schultsz, Constance; Ngo, Thi Hoa|
|Source||Journal of Antimicrobial Chemotherapy 74 (2019)3. - ISSN 0305-7453 - p. 561 - 570.|
|Department(s)||Bacteriology & Epidemiology|
|Publication type||Refereed Article in a scientific journal|
OBJECTIVES: To investigate the risk of colonization with ESBL-producing Escherichia coli (ESBL-Ec) in humans in Vietnam associated with non-intensive chicken farming. METHODS: Faecal samples from 204 randomly selected farmers and their chickens, and from 306 age- and sex-matched community-based individuals who did not raise poultry were collected. Antimicrobial usage in chickens and humans was assessed by medicine cabinet surveys. WGS was employed to obtain a high-resolution genomic comparison between ESBL-Ec isolated from humans and chickens. RESULTS: The adjusted prevalence of ESBL-Ec colonization was 20.0% (95% CI 10.8%-29.1%) and 35.2% (95% CI 30.4%-40.1%) in chicken farms and humans in Vietnam, respectively. Colonization with ESBL-Ec in humans was associated with antimicrobial usage (OR = 2.52, 95% CI = 1.08-5.87) but not with involvement in chicken farming. blaCTX-M-55 was the most common ESBL-encoding gene in strains isolated from chickens (74.4%) compared with blaCTX-M-27 in human strains (47.0%). In 3 of 204 (1.5%) of the farms, identical ESBL genes were detected in ESBL-Ec isolated from farmers and their chickens. Genomic similarity indicating recent sharing of ESBL-Ec between chickens and farmers was found in only one of these farms. CONCLUSIONS: The integration of epidemiological and genomic data in this study has demonstrated a limited contribution of non-intensive chicken farming to ESBL-Ec colonization in humans in Vietnam and further emphasizes the importance of reducing antimicrobial usage in both human and animal host reservoirs.