Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 548369
Title Black spot partial resistance in diploid roses : QTL discovery and linkage map creation
Author(s) Yan, M.; Byrne, D.H.; Klein, P.E.; Weg, W.E. van de; Yang, J.; Cai, L.
Source Acta Horticulturae 1232 (2019). - ISSN 0567-7572 - p. 135 - 141.
DOI https://doi.org/10.17660/ActaHortic.2019.1232.21
Department(s) Plant Breeding
PE&RC
EPS
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) Consensus map - Genotyping-by-sequencing - Phenotyping - QTL - SNP
Abstract

Black spot disease (Diplocarpon rosae) is the most important leaf disease of garden roses in warm humid areas. Although partial resistance to black spot has been shown to be moderately heritable, the responsible quantitative trait loci (QTL) remain unidentified. Because of the interspecific nature and high heterozygosity in commercial roses, as well as the relatively small research input compared to row crops, the genomic resources available for rose are limited. To effectively identify markers associated with QTL controlling black spot resistance, abundant markers across the genome and careful phenotyping are required. Fifteen inter-related diploid rose populations with black spot resistant cultivar R. wichuraiana ‘Basye’s Thornless’ in the genetic background were assessed based on the percent of total foliage covered with lesions in June, October and November of 2016 in College Station. Broad sense heritability was estimated at 0.51 which indicates black spot partial resistance is a moderately heritable trait. Genotyping-by-sequencing technology was used to generate SNP markers for linkage map construction. Previous anchor SSR markers were used to designate the linkage group number. The final consensus map used for black spot QTL detection contained 791 SNP covering 430 cM with the biggest gap being 6.6 cM on LG4. One major black spot QTL was discovered on LG3 explaining ~13% of the total phenotypic variance (equaling approximately 26% of the genetic variation) using pedigree-based analysis among all fifteen populations.

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