Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 548914
Title Cost-effective production of recombinant peptides in Escherichia coli
Author(s) Gaglione, Rosa; Pane, Katia; Dell'Olmo, Eliana; Cafaro, Valeria; Pizzo, Elio; Olivieri, Giuseppe; Notomista, Eugenio; Arciello, Angela
Source New Biotechnology 51 (2019). - ISSN 1871-6784 - p. 39 - 48.
DOI https://doi.org/10.1016/j.nbt.2019.02.004
Department(s) Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2019
Availibility Full text available from 2020-07-25
Keyword(s) Apolipoprotein A-I - Apolipoprotein B - Auto-inducing expression procedure - Cost-effective recombinant production - Host defence peptides - Techno-economic analysis
Abstract

Among bioactive peptides, cationic antimicrobial peptides (AMPs), also referred to as host defence peptides (HDPs), are valuable tools to treat infections, being able to kill a wide variety of microbes directly and/or modulate host immunity. HDPs have great therapeutic potential against antibiotic-resistant bacteria, viruses and even parasites. However, high manufacturing costs have greatly limited their development as drugs, thus highlighting the need to develop novel and competitive production strategies. Here, a cost-effective procedure was established to produce the high amounts of peptides required for basic and clinical research. Firstly, a novel culture medium was designed, which was found to support significantly higher cell densities and recombinant expression levels of peptides under test compared to conventional media. The procedure has been also efficiently scaled up by using a 5 L fermenter, while the costs have been lowered significantly by developing a successful auto-induction strategy, which has been found to support higher yields of target constructs and cell biomass compared to conventional strategies based on expression induction by IPTG. Interestingly, it was estimated that by increasing production scale from 100 to 1000 mg/batch, unit costs decreased strongly from 253 to 42 €/mg. These costs appear highly competitive when compared to chemical synthesis strategies. Altogether, the data indicate that the strategy represents an important starting point for the future development of large-scale manufacture of HDPs.

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