Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 549622
Title A remote cis-regulatory region is required for nin expression in the pericycle to initiate nodule primordium formation in medicago truncatula
Author(s) Liu, Jieyu; Rutten, Luuk; Limpens, Erik; Molen, Tjitse Van Der; Velzen, Robin Van; Chen, Rujin; Chen, Yuhui; Geurts, Rene; Kohlen, Wouter; Kulikova, Olga; Bisseling, Ton
Source The Plant Cell 31 (2019)1. - ISSN 1040-4651 - p. 68 - 83.
DOI https://doi.org/10.1105/tpc.18.00478
Department(s) Laboratory of Molecular Biology
EPS
Biosystematics
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

The legume-rhizobium symbiosis results in nitrogen-fixing root nodules, and their formation involves both intracellular infection initiated in the epidermis and nodule organogenesis initiated in inner root cell layers. NODULE INCEPTION (NIN) is a nodule-specific transcription factor essential for both processes. These NIN-regulated processes occur at different times and locations in the root, demonstrating a complex pattern of spatiotemporal regulation. We show that regulatory sequences sufficient for the epidermal infection process are located within a 5 kb region directly upstream of the NIN start codon in Medicago truncatula. Furthermore, we identify a remote upstream cis-regulatory region required for the expression of NIN in the pericycle, and we show that this region is essential for nodule organogenesis. This region contains putative cytokinin response elements and is conserved in eight more legume species. Both the cytokinin receptor 1, which is essential for nodule primordium formation, and the B-type response regulator RR1 are expressed in the pericycle in the susceptible zone of the uninoculated root. This, together with the identification of the cytokinin-responsive elements in the NIN promoter, strongly suggests that NIN expression is initially triggered by cytokinin signaling in the pericycle to initiate nodule primordium formation.

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