Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 549759
Title Robust sampling and preservation of DNA for microbial community profiling in field experiments
Author(s) Groenenboom, Anneloes E.; Smid, Eddy J.; Schoustra, Sijmen E.
Source BMC Research Notes 12 (2019)1. - ISSN 1756-0500
DOI https://doi.org/10.1186/s13104-019-4187-2
Department(s) Laboratory of Genetics
VLAG
Food Microbiology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2019
Keyword(s) DNA stabilisation - Fermentation - Field trial - Filter paper disks - Microbial community - Milk
Abstract

Objective: Stabilising samples of microbial communities for DNA extraction without access to laboratory equipment can be a challenging task. In this paper we propose a method using filter paper disks for the preservation of DNA from diverse microbial communities which are found in a fermented milk product. Results: Small adaptations to the DNA extraction method used for liquid fermented milk delivered DNA of sufficient amounts and quality to be used for later analyses, e.g. full community 16S amplicon sequencing. The microbial community structure obtained via the filter paper method showed sufficient resemblance to the structure obtain via the traditional DNA extraction from the liquid milk sample. This method can therefore successfully be used to analyse diverse microbial communities from fermented milk products from remote areas.

Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.