Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 549875
Title Molecular quantification of Plasmodium parasite density from the blood retained in used RDTs
Author(s) Robinson, Ailie; Busula, Annette O.; Muwanguzi, Julian K.; Powers, Stephen J.; Masiga, Daniel K.; Bousema, Teun; Takken, Willem; Boer, Jetske G. de; Logan, James G.; Beshir, Khalid B.; Sutherland, Colin J.
Source Scientific Reports 9 (2019)1. - ISSN 2045-2322
DOI https://doi.org/10.1038/s41598-019-41438-0
Department(s) PE&RC
Laboratory of Entomology
Publication type Refereed Article in a scientific journal
Publication year 2019
Abstract

Most malaria-endemic countries are heavily reliant upon rapid diagnostic tests (RDT) for malaria case identification and treatment. RDT previously used for malaria diagnosis can subsequently be used for molecular assays, including qualitative assessment of parasite species present or the carriage of resistance markers, because parasite DNA can be extracted from the blood inside the RDT which remains preserved on the internal components. However, the quantification of parasite density has not previously been possible from used RDT. In this study, blood samples were collected from school-age children in Western Kenya, in the form of both dried blood spots on Whatman filter paper, and the blood spot that is dropped into rapid diagnostic tests during use. Having first validated a robotic DNA extraction method, the parasite density was determined from both types of sample by duplex qPCR, and across a range of densities. The methods showed good agreement. The preservation of both parasite and human DNA on the nitrocellulose membrane inside the RDT was stable even after more than one year’s storage. This presents a useful opportunity for researchers or clinicians wishing to gain greater information about the parasite populations that are being studied, without significant investment of resources.

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